NK-cell compartment restoration in relation to CMV serostatus and infection/reactivation. Stratification according to the following groups: seronegative (n = 17), seropositive (n = 18), and reactivated (n = 12) CMV recipients applies to plots (A-E). (A) Intraindividual distance (Aitchison distance) between 3 and 12 months after HSCT among stratified groups based on 62 manually gated cell frequencies. (B) Evolution of the CD57⁺, NKG2C⁺ CD56^dim adaptive NK cells at 1-year follow-up after HSCT stratified among the groups fitted using LOESS regression with a 95% confidence interval. Color-coded lines represent linear regression according to the cell subset. (C) Heat map of the frequency of cell subsets at 12 months that were statistically different between CMV seronegative (green) and CMV reactivated (blue) recipients. Heat map rows display cell subsets and are colored by the z score normalized per row. Each column represents the recipient. (D) Granzyme B expression (MFI) in KIR2DL2L3S2⁺ NKG2C⁺ CD56^dim NK cells among stratified groups at 3 and 12 months after HSCT. (E) Frequency of CD107a⁺ CD56^dim NK cells in stratified groups: seronegative (n = 17), seropositive (n = 18), and reactivated (n = 12). Box plots display medians and IQRs, with whiskers representing 1.5× IQR. Wilcoxon rank-sum test in panels A,C-E. All P values were 2-sided. Statistical thresholds: ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. MFI, mean flurorescence intensity.