TCR diversity restricted during the first year after HSCT. (A) Evolution of TCR-productive Simpson clonality before allo-HSCT (t0, n = 53) in donors and at serial time points t3 (n = 54), t6 (n = 52), t9 (n = 47), and t12 (n = 48) after allo-HSCT in recipients. (B) Fractal clonal size organization defined by the productive frequency of clones at the indicated time points (t0, n = 53; t3, n = 54; t6, n = 52; t9, n = 47; and t12, n = 48). Each bar represents a single individual. The color-coded legend bar represents stratification according to the productive frequency of the individual clone. (C) TCR repertoire overlaps between donor and recipient and between post-HSCT time points in recipients calculated using the Morisita-Horn index. Morisita-Horn indices vary between 0 (no overlap) and 1 (complete overlap) and are represented along the y-axis. Box plots display medians and IQRs, with whiskers representing 1.5× IQR. Wilcoxon rank-sum test with false discovery rate correction in panel A. Kruskal-Wallis test and post hoc Dunn test in panel C. All P values were 2-sided. Statistical thresholds: ∗∗∗∗P < .0001. NS, not significant.
