American Society of Hematology

Figure 5.

PRN1008 and PRN473 block platelet activation in mouse whole blood. (A) Mouse washed platelets (2 × 108/mL) were incubated with vehicle (0.02% DMSO) or indicated concentration (0.5, 2, 5, or 10 μM) of Btk inhibitors PRN1008 for 1 hour. Platelet activation, indicated by activated integrin αIIbβ3 (JON/A) and P-selectin surface expression, was then assessed by flow cytometry in response to stimulation with snake venom toxin rhodocytin 100 (Ai) and 300 nM (Aii) or CRP 3 (Bi) and 10 μg/mL (Bii) or PAR4 receptor activating peptide (100 or 500 nM) (C). Mean ± SEM (n = 3-6). Rhodo, rhodocytin.

PRN1008 and PRN473 block platelet activation in mouse whole blood. (A) Mouse washed platelets (2 × 10⁸/mL) were incubated with vehicle (0.02% DMSO) or indicated concentration (0.5, 2, 5, or 10 μM) of Btk inhibitors PRN1008 for 1 hour. Platelet activation, indicated by activated integrin αIIbβ3 (JON/A) and P-selectin surface expression, was then assessed by flow cytometry in response to stimulation with snake venom toxin rhodocytin 100 (Ai) and 300 nM (Aii) or CRP 3 (Bi) and 10 μg/mL (Bii) or PAR4 receptor activating peptide (100 or 500 nM) (C). Mean ± SEM (n = 3-6). Rhodo, rhodocytin.

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