Figure 2.
Targeting of Herc1 increases the sensitivity of murine AML cell to nucleoside analogs in vitro. (A) Representation of nucleoside analogs used for cell viability assays. (B) MA cells were seeded in quadruplicates into 96-well plates and treated with varying concentrations of Ara-C. The number of viable cells was measured after 72 hours using the CTG Luminescent Cell Viability Assay. One of the independent experiments is shown. (C) IC50 concentrations of Ara-C of 6 independent repeated experiments in MA with either sgHerc1 number 1 or sgHerc1 number 2 analyzed together or separate with sgCtrl. Significance was determined using 1-way ANOVA followed by Dunnett’s multiple comparisons test. ∗P ≤ .05. (D) MA cells were seeded in quadruplicates into 96-well plates and treated with varying concentrations of Gem. The number of viable cells was measured after 72 hours using the CTG Luminescent Cell Viability Assay. One of independent experiments is shown. (E) IC50 concentrations of Gem of 8 independent repeated experiments in MA with either sgHerc1 number 1 and sgHerc1 number 2 analyzed together or separate with sgCtrl. Significance was determined using one-way ANOVA, followed by Dunnett multiple comparisons test. ∗P ≤ .05. (F) IC50 concentrations of Flu of 8 independent repeated experiments in MA with either sgHerc1 number 1 or sgHerc1number 2, analyzed together or separate with sgCtrl. Significance was determined using a 1-way ANOVA, followed by Dunnett multiple comparisons test. ∗P ≤ .05. (G) HM cells were seeded in quadruplicates into 96-well plates and treated with varying concentrations of Ara-C. The number of viable cells was measured after 72 hours using the CTG Luminescent Cell Viability Assay. One of independent experiments is shown. (H) IC50 concentrations of Ara-C of 3 independent repeated experiments in HM. Significance was determined using a 1-way ANOVA, followed by Dunnett multiple comparisons test. ∗P ≤ .05. (I) HERC1 expression was analyzed in a panel of human AML cell lines (n = 19) and linked to their respective cytarabine IC50. Cell lines were arbitrarily split into low (n = 9) and high (n = 10) expressers of HERC1. Significance was determined using an unpaired 1-tailed t test. ∗P ≤ .05.

Targeting of Herc1 increases the sensitivity of murine AML cell to nucleoside analogs in vitro. (A) Representation of nucleoside analogs used for cell viability assays. (B) MA cells were seeded in quadruplicates into 96-well plates and treated with varying concentrations of Ara-C. The number of viable cells was measured after 72 hours using the CTG Luminescent Cell Viability Assay. One of the independent experiments is shown. (C) IC50 concentrations of Ara-C of 6 independent repeated experiments in MA with either sgHerc1 number 1 or sgHerc1 number 2 analyzed together or separate with sgCtrl. Significance was determined using 1-way ANOVA followed by Dunnett’s multiple comparisons test. ∗P ≤ .05. (D) MA cells were seeded in quadruplicates into 96-well plates and treated with varying concentrations of Gem. The number of viable cells was measured after 72 hours using the CTG Luminescent Cell Viability Assay. One of independent experiments is shown. (E) IC50 concentrations of Gem of 8 independent repeated experiments in MA with either sgHerc1 number 1 and sgHerc1 number 2 analyzed together or separate with sgCtrl. Significance was determined using one-way ANOVA, followed by Dunnett multiple comparisons test. ∗P ≤ .05. (F) IC50 concentrations of Flu of 8 independent repeated experiments in MA with either sgHerc1 number 1 or sgHerc1number 2, analyzed together or separate with sgCtrl. Significance was determined using a 1-way ANOVA, followed by Dunnett multiple comparisons test. ∗P ≤ .05. (G) HM cells were seeded in quadruplicates into 96-well plates and treated with varying concentrations of Ara-C. The number of viable cells was measured after 72 hours using the CTG Luminescent Cell Viability Assay. One of independent experiments is shown. (H) IC50 concentrations of Ara-C of 3 independent repeated experiments in HM. Significance was determined using a 1-way ANOVA, followed by Dunnett multiple comparisons test. ∗P ≤ .05. (I) HERC1 expression was analyzed in a panel of human AML cell lines (n = 19) and linked to their respective cytarabine IC50. Cell lines were arbitrarily split into low (n = 9) and high (n = 10) expressers of HERC1. Significance was determined using an unpaired 1-tailed t test. ∗P ≤ .05.

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