PIK3CA UP and PTEN KO leads to different sensitivities to BH3 mimetics. (A-F) Number of apoptotic cells 24 hours after exposure to the BH3 mimetics venetoclax (0.01, 0.1, and 1 μM), S63845 (0.01, 0.1, and 1 μM), and A1155463 (0.01, 0.1, and 1 μM). Apoptosis of the treated cells was normalized to the apoptosis of the untreated cells (n = 3). (G) Western blot analysis of selected BCL2 family proteins in PIK3CA UP and PTEN KO cell lines compared with the respective control MCL cell lines (for each sample, n = 2); data are represented by means ± SD; ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001; (N) represents the number of biological replicates.