Figure 6.
CDK9 degradation reduces S276/S303-RUNX1 phosphorylation and promotes erythroid fate specification in MEPs. (A-C) Representative images of intracellular staining of pS276-RUNX1, pS303-RUNX1, and total RUNX1 (left) in DMSO vs 500 nM Palbociclib (PD 0332991, PD) for 48 hours (A), DMSO vs 50 nM flavopiridol (FP) for 2 hours (B), and DMSO vs 500 nM THAL-SNS-032 (THAL) for 16 hours (C). Bars show average ± SD MFI after normalization to the MFI of DMSO in each experiment (right). Dots of the same shape represent 1 experiment (from the same donor, processed in parallel). (D) Effects on colony formation of MEPs treated with DMSO or 500 nM THAL-SNS-032 for 48 hours. Average ± SD shown (n = 3). (E) Colony formation of MEPs transduced to express RUNX1-WT or RUNX1-4D, with or without 500 nM THAL treatment for 48 hours. For all plots, ∗P < .05, ∗∗P < .01.

CDK9 degradation reduces S276/S303-RUNX1 phosphorylation and promotes erythroid fate specification in MEPs. (A-C) Representative images of intracellular staining of pS276-RUNX1, pS303-RUNX1, and total RUNX1 (left) in DMSO vs 500 nM Palbociclib (PD 0332991, PD) for 48 hours (A), DMSO vs 50 nM flavopiridol (FP) for 2 hours (B), and DMSO vs 500 nM THAL-SNS-032 (THAL) for 16 hours (C). Bars show average ± SD MFI after normalization to the MFI of DMSO in each experiment (right). Dots of the same shape represent 1 experiment (from the same donor, processed in parallel). (D) Effects on colony formation of MEPs treated with DMSO or 500 nM THAL-SNS-032 for 48 hours. Average ± SD shown (n = 3). (E) Colony formation of MEPs transduced to express RUNX1-WT or RUNX1-4D, with or without 500 nM THAL treatment for 48 hours. For all plots, ∗P < .05, ∗∗P < .01.

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