RUNX1 chromatin association is not significantly affected by RUNX1-4A and RUNX1-4D. (A) STREME (simple, thorough, rapid, enriched motif elicitation) motif enrichment analysis showing that CUT&RUN binding sites for RUNX1-4A, RUNX1-WT, and RUNX1-4D are highly enriched for the conserved RUNX1 binding motif, as well as ETS and GATA binding motifs. (B) Distance to the nearest TSS for RUNX1-4A (4A), RUNX1-WT (WT), and RUNX1-4D (4D) binding sites. (C) Differential binding analysis of RUNX1 mutants compared with WT (4A: left, 4D: middle). Cutoff for significance was adjusted P < .05 (horizontal dashed line), and log₂ fold change (log2FC) of less than −1 or >1 (vertical dashed lines). Log₂ fold change of <0 indicates that RUNX1-WT peak is higher than that of RUNX1-4A or RUNX1-4D. Differential binding analysis of RUNX1-4A vs RUNX1-4D (right) shows 127 peaks beyond the significance cutoff in red.