Figure 2.
MkPs and ErPs have similar RUNX1 RNA and protein levels, but pS-RUNX1 levels are highest in MkPs. (A) DESeq2-normalized counts between CMPs, MEPs, ErPs, and MkPs were compared from 6 biological replicates (4 for MkPs). Dots of the same shape represent data from the same donor. (B,D,F) Representative images of intracellular staining with antibody targeting total RUNX1 protein (B), pS276-RUNX1 (D), and pS303-RUNX1 (F) with unstained cells used as controls. Dashed line is the cutoff for positive staining. (C,E,G) y-axes: the MFI for each population after normalization to the MFI of CMPs in each experiment. Dots of the same shape represent 1 experiment (from the same donor, processed in parallel). Average ± SD shown (∗P < .05). ns, not significant.

MkPs and ErPs have similar RUNX1 RNA and protein levels, but pS-RUNX1 levels are highest in MkPs. (A) DESeq2-normalized counts between CMPs, MEPs, ErPs, and MkPs were compared from 6 biological replicates (4 for MkPs). Dots of the same shape represent data from the same donor. (B,D,F) Representative images of intracellular staining with antibody targeting total RUNX1 protein (B), pS276-RUNX1 (D), and pS303-RUNX1 (F) with unstained cells used as controls. Dashed line is the cutoff for positive staining. (C,E,G) y-axes: the MFI for each population after normalization to the MFI of CMPs in each experiment. Dots of the same shape represent 1 experiment (from the same donor, processed in parallel). Average ± SD shown (∗P < .05). ns, not significant.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal