Evaluation of gene expression and spleen-to-body weight ratios in WT, AG-KO, and AG-KO^ALS20αIrecipients. (A) EPO gene expression was measured in kidney tissue of WT (n = 12), AG-KO (n = 14), and AG-KO^ALS20αI (n = 7) animals. (B) Hepcidin (HAMP) gene expression was measured in liver tissue of WT (n = 12), AG-KO (n = 14), and AG-KO^ALS20αI (n = 6) animals. (C) Ratio of the spleen-to-body weight measured in WT (n = 2), AG-KO (n = 6), and AG-KO^ALS20αI (n = 7). The mean and standard error of the mean are indicated. Significant differences between groups were measured using a Kruskal-Wallis test with Dunns multiple comparisons test: ∗P ≤ .05; ∗∗P ≤ .01; ∗∗∗P ≤ .001; ∗∗∗∗P ≤ .0001.