HDAC3 inhibition restores MHC expression in MM cell lines. (A) Immunoblot of H3K27ac 24 hours after treatment with 10 μM RGFP966. (B) Heat map of z scores for all expressed MHC genes in KDM6A clonal isogenic cell lines. (C) mRNA analysis by qPCR normalized to GAPDH 24 hours after treatment with 10 μM RGFP966 (3-5 biological replicates; ± SD. Wilcoxon t test). (D) Flow cytometry analysis of HLA-A/B/C and HLA-DM/DQ/DR in myeloma isogenic cell lines treated or not with 10 μM RGFP966 for 24 hours or 72 hours. Panel on the right shows the histograms MFI quantification of HLA-A/B/C and HLA-DM/DQ/DR (4 or 5 biological replicates; ± SD, Wilcoxon t test). Ctl, control.
Figure 5.

HDAC3 inhibition restores MHC expression in MM cell lines. (A) Immunoblot of H3K27ac 24 hours after treatment with 10 μM RGFP966. (B) Heat map of z scores for all expressed MHC genes in KDM6A clonal isogenic cell lines. (C) mRNA analysis by qPCR normalized to GAPDH 24 hours after treatment with 10 μM RGFP966 (3-5 biological replicates; ± SD. Wilcoxon t test). (D) Flow cytometry analysis of HLA-A/B/C and HLA-DM/DQ/DR in myeloma isogenic cell lines treated or not with 10 μM RGFP966 for 24 hours or 72 hours. Panel on the right shows the histograms MFI quantification of HLA-A/B/C and HLA-DM/DQ/DR (4 or 5 biological replicates; ± SD, Wilcoxon t test). Ctl, control.

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