Facilitation of SIPA and its regulation by membrane cholesterol. (A) SIPA observed after WB and PRP were exposed to different shear rates for 5 minutes at 37°C (n = 6 per condition; 2-way ANOVA). (B) Viscosities of WB and PRP under different shear rates (n = 88; 46% male; Mann-Whitney rank sum test). (C) SIPA in WB and PRP exposed different shear stresses for 5 minutes at 37°C (n = 5-26 per condition; 2-way ANOVA). (D) SIPA of platelets reconstituted with fresh, partially fixed (0.03% glutaraldehyde at 5 minutes), and completely fixed (0.1% glutaraldehyde at 1 hour) RBCs (n = 4 per condition; 1-way ANOVA, P = .0016). (E) MβCD (10 mM) treated and untreated RBCs were drawn through microfluidic channels under negative pressure (2 psi; bar = 20 μm) and their movements were recorded at 2000 fps. RBC deformability was quantified by Taylor deformation (top panel: deformation distributions at the entrance, middle, and exit of the constriction, representative of 5 independent runs). (F) Shear-induced aggregation of platelets reconstituted with MβCD-treated RBCs (n = 6-8 per condition; 1-way ANOVA, P < .0001). fps, frames per second.