Multidimensional analysis and heat map with unsupervised clustering of caspase-1/4/5 activity, speck formation, and inflammasome-associated biomarkers. (A) Principal component analysis incorporating inflammasome components (caspase-1/4/5 activity, FLICA⁺ ASC-speck formation) and 11 inflammatory biomarkers (ferritin, CRP, IFN-γ, IL-6, IL-10, IL-13, IL-18, IL18BP, CXCL9, CD163, and TNF-α) demonstrates significant overlap in clusters when defined by clinical syndrome. Individuals are represented by small colored circles for each group, whereas the overall group is represented by large colored circles. Participants with MCD and concomitant PEL are depicted as red circles with a blue outline. (B) Heat map with unsupervised clustering of inflammasome components, KSHV-VL (copies per million PBMCs), and monocyte-associated biomarkers identified that those with MCD primarily cluster together, including those with concomitant PEL (depicted with blue circle inside red box). Those with KS alone form a separate distinct cluster. Patients with KICS are distributed throughout. Individuals with MCD, PEL, and KICS may or may not have had concomitant KS. Analysis performed in R (version 4.1.2) using the FactoMineR, factoextra, and heatmap packages.