Flowchart describing how VWF antibodies are detected and characterized through multiple laboratory methods. VWF antibody assessment strategy used to detect, quantify, and characterize anti-VWF IgG and IgM antibodies in type 3 VWD plasma and control samples using a systematic ELISA-based approach. Plasma samples are screened and confirmed using 95th percentile thresholds (95%) as determined by the healthy control cohort. VWF antibodies in the positive samples are further assessed for concentration in A.U. of immunoglobulin (A.U. Ig), neutralization of VWF function as tested via mixing studies with VWF:CB3, VWF:CB4, VWF:F8B, and VWF:GPIbM activity ELISAs, and IgG subclass if applicable.