The function of monocyte-exosome. (A) Cell viability of MM cells in response to treatment with CL-Exo. (B-C) The CL-Exo treatment at the IC50 concentration induced apoptosis (B) and cell cycle arrest (C) in MM cells. (D) The relative mRNA expression level of IL-1β and CD206 in macrophage after treated with 100 μg/mL CL-Exo. (E) Cell viability of NK-92 cells in response to treatment with 100 μg/mL CL-Exo. (F) The CL-Exo treatment at a concentration of 100 μg/mL enhanced the antitumor activity of NK-92 cells to MM cells. (G) The calcium deposition and ALP activity was determined after 7-day and 14-day treatment of 100 μg/mL CL-Exo in MSCs. Scale bar, 100 μm. (H) The relative mRNA expression levels of Runx2, OPN, OCN, ALP, and Col-l were determined by qPCR after 7-day and 14-day treatment of 100 μg/mL CL-Exo in MSCs. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. ALP, alkaline phosphatase ; IL-1β, interleukin-1β; mRNA, messenger RNA; n.s, no significance; qPCR, quantitative real-time PCR.