CPI203 and quisinostat support expansion of functional HSCs. (A) Schematic overview of primary transplantation. A total of 25 000 and 50 000 nucleated expanded cells in the indicated culture conditions were transplanted per mouse. (B) Human engraftment in the BM of NSG mice at week 20 after transplantation (n = 5 mice per group; mean ± standard error of the mean [SEM]; ∗P < .05). Statistical significance was calculated by 1-way ANOVA, with multiple comparisons. (C) Representative fluorescence-activated cell sorter (FACS) plots of huCD45 in NSG BM 20 weeks after transplantation. (D) Kinetics of chimerism in PB over time (n = 5 mice per group; significance of STF vs STF + CPI203 is shown in the plot). (E) Human engraftment in different organs 20 weeks after transplantation (n = 5 mice per group). (F) Frequency of different populations in BM (n = 5 mice per group). (G) Frequency of different populations in the Thy (n = 5 mice per group; mean ± SEM). Statistical significance was calculated by 2-way ANOVA with multiple comparisons: ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.