Quisinostat increases LV transduction efficiency. (A) Schematic experimental design. CD34⁺ cells were transduced with LV-GFP 24 hours after stimulation in cytokine-supplemented medium alone or the presence of quisinostat (0.5 nM) or CPI203 (150 nM). Cells were harvested for flow cytometry analysis, DNA isolation, and reverse transcription polymerase chain reaction (RT-PCR), 4 days after transduction. (B) Frequency of GFP⁺ 4 days after transduction in live cells (n = 9; mean ± SD). (C) VCN was determined by RT-PCR (n = 3; mean ± SD; ∗P < .05; unpaired t test). (D) Frequency of transduced HSCs and MPPs in the indicated culture conditions are shown (n = 9; mean ± SD). (E) Cell counts of transduced HSCs and MPPs (n = 9; mean ± SD). (F) The plot shows the mean fluorescence intensity of H3K27ac in different populations of expanded cells in the indicated culture conditions (n = 3; mean ± SD). Statistical significance was determined by 2-way ANOVA test with multiple comparisons: ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. H3K27ac, acetylation level of lysine 27 on histone 3.