Foxo1^iLKO mice display disturbed iron metabolism under both ND and HID.Foxo1^iLKO and Foxo1^iCon mice were maintained on an ND or a 2-week HID before they were euthanized at age 8 weeks. Iron-related phenotypes were assessed. (A) Serum hepcidin levels. (B) Liver hepcidin (Hamp1) mRNA expressions. (C) Liver nonheme iron concentrations. (D) Liver Hamp1 mRNA/liver nonheme iron concentration ratios (Hamp1/iron). (E) Spleen nonheme iron concentrations. (F) Pancreas nonheme iron concentrations. (G) Heart nonheme iron concentrations. (H) Serum iron levels. (I) Transferrin saturation levels. (J) Perls iron staining of the liver and spleen tissues (scale bar, 100 μm). ♂, male mice; ♀, female mice; n = 5 to 7 in each group. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. The P value was calculated by the 1-way ANOVA with the Sidak multiple comparison test in sex- and diet-matched data.