Divergent dynamic changes of CD8⁺ BM T cells. (A-B) UMAP (A) and box plots (B) depicting 2 predicted developmental trajectories inferred via Slingshot. In the UMAP plot cells are colored according to the pseudotime and the 2 trajectories are labeled based on the terminal cell state (Term/SenL and Act). (C) Line plots showing the smoothed gene expression of genes that are differentially expressed (patternTest) over the different pseudotime trajectories defined in panels A and B. (D) Heatmap of smoothed gene expression of the DEGs obtained using patternTest along the 2 pseudotime trajectories. Depicted on the right are selected genes among the differentially expressed. (E-F) UMAP colored by the annotated CD8⁺ subsets overlaid with the predicted RNA velocity stream (E) and with inferred latent time trajectories (F) obtained using partition-based graph abstractions (PAGA).