Characterization of CD8⁺ BM T cells in AML at the transcriptional level. (A) Dot plot of the average expression of selected genes across the CD8⁺ clusters. (B) Feature plots of selected CD8⁺ T-cell gene markers. (C) Heat map of DEGs showing the top 10 DEGs for each cluster. (D) Heatmap showing the Spearman correlation of log-normalized counts of the top 500 most variable genes determined by Seurat. Selected genes with a correlation coefficient above the 99th percentile threshold are highlighted on the right. (E) Uniform manifold approximation and projection (UMAP) plot of the distribution of CD8⁺ T cells colored by selected DEGs. (F) Barplot illustrating the relative frequencies of ProjecTILs states within each of the annotated subsets (C1, C2, C3, and C4). (G) Schematic pipeline used to identify Term/SenL signature using bulk-RNAseq. (H) Feature plot of CD8⁺ T-cell senescence (left) and dysfunction (right) scores. (I) Pearson correlation of the senescence (x-axis) and dysfunction (y-axis) scores. (J) Distribution of the averaged expression (y-axis) of senescence (top) and dysfunction (bottom) scores across the CD8⁺ clusters. DEGs, differentially expressed genes; Max, maximum; Min, minimum.