CAR-37 T-cell expansion after infusion. (A) The absolute numbers of neutrophils, monocytes, and lymphocytes per microliter of blood after CAR-37 infusion (day 0) are shown. (B) Flow cytometry dot plots of CD3⁺EGFR⁺ CAR-37 T cells in peripheral blood are shown on day −5 (before CAR-37 T-cell infusion) and day 14 after CAR-37 T-cell infusion. (C) The percentage of CD3⁺ cells that were EGFR⁺ as detected in patient peripheral blood by flow cytometry over time after CAR-37 T-cell infusion on day 0 is shown. (D) The number of CAR-37 T cells per microliter of peripheral blood is shown as calculated from the percentage of CD3⁺EGFR⁺ cells detected by flow cytometry and the absolute lymphocyte count (left y-axis, closed symbols), as well as the VCN, reported as copies per microgram of DNA (right y-axis, open symbols), over time after CAR-37 T-cell infusion. PE, phycoerythrin.