XIAP-deficient cells exhibit defective autophagic flux upon serum starvation. Immunoblots and densitometry of LC3 protein from (A) PBMC of proband and his healthy father and (B) control and XIAP-KO THP-1 cells after starvation for the indicated time. GAPDH loading control was used for normalization of LC3-II. (C) Representative immunofluorescence images showing LC3 staining in control and XIAP-KO THP-1 cells at baseline and after 30 minutes of starvation and quantification of average increase in number of LC3 puncta upon starvation. Scale bars represent a distance of 10 μm. Results are representative of 3 independent experiments. Graphs indicate the mean and standard error of the mean.