Impact of cumulative DSA-MFI on 2-hour CCI. (A) Violin plots showing 2-hour posttransfusion CCI (x-axis) by ranges of cumulative DSA-MFI, RAP transfusions, or transfusions with no donor HLA mismatches (y-axis). The horizontal dashed line denotes CCI = 7500. Horizontal bars for each violin plot denote the median for each group, and n for each plot denotes the number of transfusions in each group. ∗Denotes P < .05 in 2-tailed t test compared against 4/4 HLA matched or antigen-negative transfusions. (B) Estimated population mean 2-hour CCI at various values for total MFI conditional on all other predictors included at the linear mixed effects model at their median values; ∗95% confidence band calculated using bootstrap resampling of participants. (C) Association between cumulative DSA-MFI (x-axis) and cumulative C1q MFI compared across 282 transfusions (C1q testing on 58 patient samples). Both axes are log₁₀ scaled. Points are colored by adequate (blue, ≥7500) or inadequate (red, <7500) 2-hour posttransfusion CCI. (D) Mean 2-hour posttransfusion CCI with 95% confidence interval (x-axis) across given ranges of cumulative DSA-MFI by number of mismatched donor HLAs for each DSA-MFI stratum (y-axis).