Anti-c-Kit/CD47 plus oral ruxolitinib enables fully mismatched murine allogeneic transplantation with high-level multilineage engraftment. (A) Experimental schema for BALB/c→B6 allo-HSCT. (B-C) Longitudinal analysis of donor chimerism by cell lineage (B) and complete blood count (C) in mice that received transplantation. In panel B, the dotted lines represent donor chimerism for individual mice and solid lines are means. In panel C, the horizontal dotted lines indicate the lower end of the reference range for each cell count. (D) Body weight and graft-versus-host disease clinical scoring during the peritransplant period after conditioning and HSCT as per panel (A). (E) Hematoxylin and eosin staining of representative formalin-fixed paraffin-embedded sections (from n = 2 recipient mice) of dorsal skin, small intestine, colon, and liver 45 days after HSCT from mice conditioned and transplanted as per panel A (original magnification ×200). Histology slides were viewed with an Accu-Scope EXC-120 light microscope (10× eyepiece with 18× mm field of view and 20× objective/0.40 numerical aperture, 20°C in air). (F) Successful BALB/c→B6 HSCT recipients or nontransplanted B6 mice were surgically engrafted with skin from BALB/c mice. Photographs of panels E and F were taken using an Apple iPhone 15. Numerical data are presented as mean ± standard error of the mean. Two-way analysis of variance for repeated measures was used in panel B; ∗∗∗∗P < .0001. BM, bone marrow; WBC, white blood cell count.