PS exposure potentiates fibrin accumulation around the platelet plug. (A-B) Surface view for accumulation of PS (red [aV]; left panel), fibrin (blue; right panel), and platelets (gray) at the indicated time points during hemostatic plug formation in WT (A), and CypD^−/−mice (B). (C-E) SFI ± SEM over time for PS (CypD^−/−, n = 7 injuries in 3 mice; and WT, n = 8 injuries in 3 mice) (C), fibrin (CypD^−/−, n = 27 injuries in 3 mice; and WT, n = 19 injuries in 3 mice) (D), and platelets (CypD^−/−, n = 27 injuries in 3 mice and WT, n = 20 injuries in 3 mice) (E). Same WT controls as in Figure 2. Statistical significance was determined for fluorescence signals of the last 3D stack acquired during the observation period. ns, not significant; ∗P < .01; ∗∗∗P < .001. Scale bar indicates 50 μm.