Plasminogen rapidly localizes to fibrin ring during hemostatic plug formation. Mice were injected with anti–fibrin-AF488 antibody, anti–GPIX-AF568 antibody, and AF647-conjugated, catalytically inactive rPlg. (A-B) Representative images (intravascular view) for fibrin (blue), platelets (gray), and rPlg (red) staining at the indicated time points after laser injury in control (A) or Plg^−/− mice (B). Scale bar indicates 50 μm.