Expression of LSC markers in AML. (A) Pearson correlation analysis of genes encoding known AML LSC markers in 3 independent cohorts. Color and size represent the direction and the magnitude of the correlation, respectively. Only correlations with P < .05 are shown. (B) Scatter plots showing the relationship between JAM3 and GPR56 mRNA expression for individual samples of the indicated cohort. Pearson correlation and P value are shown. (C) Uniform manifold approximation and projection (UMAP) plots of flow cytometry results showing expression of the indicated markers by JAM-C–expressing cells obtained from 62 concatenated samples; 7258 cells are shown. (D) Histogram showing the frequency of cells expressing JAM-C (left panel) or GPR56 (right panel) within the indicated phenotypic compartment defined by CD34 and CD38 expression. (E) Histogram showing the frequency of JAM-C–expressing cells within the indicated phenotypic compartment defined by combination of GPR56 and CD34 expression as described in Pabst et al.17 Data are represented with mean ± standard error of the mean (SEM); ns, not significant; ∗P < .05; ∗∗P < .01; ∗∗∗P < .001.
