MDF VVPs administered intranodally induce potent and prolonged B-cell depletion in NHPs. (A) Activation of NHP T cells in the blood 7 days after VivoVec administration, as measured by CD25. (B) Anti-CD20 CAR expression (Flag-tag) on NHP CD3⁺ T cells in the blood 10 days after VivoVec administration. (C) Percent of NHP T cells expressing CAR (top), overall numbers of CAR positive T cells (middle), and total B cells in the blood (bottom) over time. (D) Representative flow plots of B-cell depletion in Z20106. (E) Biodistribution of VivoVec CAR payload in various tissues 139 days after particle administration in Z20106, measured by ddPCR. VCN, vector copy number.