Personalized neoantigen cancer vaccine identification and prioritization. (A) Swarm plots display the number of vaccine candidates on the y-axis for the entire cohort at each stage of filtering (x-axis), moving from left to right. The bar graphs depict the numbers of final neoantigen vaccine candidates for each patient, colored according to source (SNV, indel, BCR, and fusion) and are sorted from least to most total candidates. Bar graph (B) includes nonneoantigen mutations that did not pass neoantigen filtering, whereas bar graph (C) only contains final candidates. The red line in panel C depicts the minimum cutoff of 2 candidates required for potential vaccine design.