Clonality analysis of BCR populations within healthy normal samples and FL samples. The plots show the composition of BCR repertoire of both normal (left half) and malignant (right half) samples for each of Ig chains separately: panels A-C correspond to heavy (blue), kappa (orange), and lambda (green) chains, respectively. In each panel, the upper histogram shows the coverage of the given Ig chain in the sample (log10 of read counts), whereas the lower stacked bar plot shows BCR repertoire structure. Each bar from bottom to top is composed of 10 dark sections representing the fraction of repertoire for the 10 largest clones in the sample and a single top light gray section representing all other (minor) clones. The colored bottom sections depict the single most dominant clone that exceeded the cutoff value of fraction in the sample (9%) and had sufficient overall coverage (>40 reads for malignant samples whereas normal samples were preselected having >100 reads for each chain, see “Methods”). When both light chains passed the cutoff, only the one with the larger fraction was selected and colored. Stars below each panel indicate major clonotypes predicted to result in one or more high-quality neoantigen vaccine candidates.
