IL-9 secreted by AML LSCs upregulates histone lysine methyltransferase genes in BM–infiltrating CD4⁺ T cells. (A) GSEA of the IL9 signaling signature in AML CD4⁺ vs CTRL CD4⁺ T cells within the entire cohort of AML samples (n = 30) or in each respective risk group. (B) GO analysis of the IL-9 regulated 88-target genes in CD4⁺ T cells. GO enrichment score of ≥3 indicates significant changes. (C) Circus plot illustrating the top 8-genes involved in several of the dysregulated pathways in the GO analysis (4B) (D) Heat map illustrating the expression of important family members of histone-lysine N-methyltransferase 2 (KMT2) complex in CD4⁺ T cell in the different AML risk groups. (E) Correlation matrix of lysine methyltransferase complex genes in AML CD4⁺ T cell. Statistics: Pearson correlation. CTRL, control; neg, negative; pos, positive.