Impaired viral clearance generates a PLC-depleting IFN-γ–mediated immune response in adult mice. (A) Representative images and overall plot of bioluminescence in 4-week-old Eμ-ret pups infected with luciferase-tagged CMV with and without NK cell depletion (and uninfected controls) at 3, 6, and 10 dpi. For NK cell depletion, 50-μg asialo GM1 antibody was administered at days –3, –1, +1, +4, and +7 (day 0 was the time of infection) (2-way ANOVA; n = 4-8; ∗∗∗∗P < .0001). (B) ELISA-based quantification of IL-12p40 (anti-p40 Ab) and IFN-γ (anti-IFN-γ Ab) in sera from PBS-injected control and CMV-infected (with and without NK depletion) adult Eμ-ret mice at 10 dpi (1-way ANOVA; n = 6-10; ∗∗P < .005; ∗∗∗∗P < .0001). (C) PLC burden in the spleens of PBS-injected control and CMV-infected (with and without NK depletion) adult Eμ-ret mice at 10 dpi (1-way ANOVA; n = 10-16; ∗P < .05; ∗∗P < .005). (D) Spleen (left panel) and BM (right panel) B-cell Fr burden after daily administration of PBS (Ctrl) or 10 μg/dose IFN-γ to 4-week-old adult Eμ-ret mice for 7 days (Mann-Whitney test; n = 6 Ctrl and 7 IFN-γ; ∗P < .005). All data are presented as mean ± SEM. Nonsignificant comparisons in all graphs are not labeled.