Exogenous IFN-γ replicates BCP Fr–specific depletion of PLC. (A) Change in cell number of flow-sorted PLC cocultured on stromal cells in the absence (Ctrl) or presence (IFN-γ) of 1-ng/mL IFN-γ for 60 hours. Quantification of PLC was achieved by Incucyte (Sartorius) live-cell analysis at 6-hour intervals. (B) PLC burden in the spleens of noninfected wild-type (R/wt) Eμ-ret pups after administration of different concentrations of IFN-γ (1, 2, or 5 μg/dose) every 2 days, from 9 to 16 days of age (1-way ANOVA; n = 3-5; ∗∗P < .005). (C) PLC burden in spleens of noninfected Stat4^–/– (R/Stat4) Eμ-ret mice injected with 5 μg/dose IFN-γ every 2 days, from 9 to 16 days of age (Mann-Whitney test; n = 9-10; ∗∗∗P < .005). (D) Spleen (left panel) and BM (right panel) B-cell Fr burden in PBS (Ctrl) and 5 μg/dose IFN-γ–injected neonatal Eμ-ret mice (Mann-Whitney test; spleen: n = 4 Ctrl and 5 IFN-γ; and BM n = 6 Ctrl and 10 IFN-γ; ∗P < .05). All data are presented as mean ± SEM. Nonsignificant comparisons in all graphs are not labeled.