Gene expression analysis indicates pathway utilization changes in PLC after neonatal MCMV infection. Selected gene sets from the Molecular Signatures Database, Molecular Hallmarks (hallmark gene sets) collection, enriched among upregulated and downregulated pathways in PLC from MCMV-infected neonates compared with PBS-injected controls (ranked according to decreasing normalized enrichment score). (A) Top 16 statistically significant upregulated biological pathways. (B) GSEA enrichment plots showing upregulation of gene sets representing IFN-α, IFN-γ, and TNF-α, and inflammatory responses. (C) Top 14 downregulated biological pathways. (D) GSEA enrichment plots showing downregulation of gene sets representing cell cycle and metabolism. ES, enrichment score; FDR, false discovery rate; NES, normalized enrichment score; NOM, nominal; size, number of genes in each set.