![Depletion of PLC is dependent on IL-12p40. (A) Enzyme-linked immunosorbent assay (ELISA)–based quantification of IL-12p40 (anti-p40 antibody [Ab]), IL-12 (anti-p70 Ab), and IL-23 (anti-p19 Ab) in sera from neonate and adult Eμ-ret mice at 3, 6, and 10 dpi (Mann-Whitney; n = 3-7). (B) Representative flow cytometry plots showing PLC in splenocytes of Eμ-ret pups after CMV infection in the presence of indicated neutralizing antibodies. (C) Cumulative results showing PLC burden in spleens of Eμ-ret pups infected along with p40, p19, and/or p70 neutralizing antibodies (1-way ANOVA; n = 4-11). (D) PLC numbers in spleens of PBS (Ctrl) or virus (CMV)-injected Rag1–/– (R/Rag, left panel), wild-type (R/wt), and NK-depleted wt (NK-dep, right panel) Eμ-ret pups at 10 dpi (Mann-Whitney; R/Rag1, n = 10 PBS and 10 MCMV; 1-way ANOVA; n = 8 PBS, 8 CMV, and 7 CMV + NK-depleted; pooled results from 3 independent experiments). All data presented as mean ± SEM (∗∗∗P < .001; ∗∗∗∗P < .0001). ns, not significant (nonsignificant comparisons in graphs A and C are not labeled).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/144/8/10.1182_blood.2024025038/1/blood_bld-2024-025038-gr3.jpeg?Expires=1727748948&Signature=ASWKFsr~eDomnPienxzzVulwKuXsAIGiV1rY9TN3SMAzYTw3vYb3OknVIC3H1xFa8UUimKec3BbsC~eyf3BTCS-0v1bDQtaT8DwIBU5HRIb~3hoGDNpN88y6sOwsW3R702fWNn5dtTMqhRiADa8nxrHSRTCFZJ9QWbl7faZKVGQewYIGKGKkPHfnebpT~vWe5im~WbsROq3EZyM9FKSGrhpBKB1Cme-J7jjmkjyxVhU~xUJ7uf8e8WNgr70o-HiOCJElQ4ahgGUhUBvnb2wuQAj9wLPY8RMtboxur8TLNGjIez3tti08MSBKMyggvewfpvfAHKB1xsR8V2VXdL4otw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Depletion of PLC is dependent on IL-12p40. (A) Enzyme-linked immunosorbent assay (ELISA)–based quantification of IL-12p40 (anti-p40 antibody [Ab]), IL-12 (anti-p70 Ab), and IL-23 (anti-p19 Ab) in sera from neonate and adult Eμ-ret mice at 3, 6, and 10 dpi (Mann-Whitney; n = 3-7). (B) Representative flow cytometry plots showing PLC in splenocytes of Eμ-ret pups after CMV infection in the presence of indicated neutralizing antibodies. (C) Cumulative results showing PLC burden in spleens of Eμ-ret pups infected along with p40, p19, and/or p70 neutralizing antibodies (1-way ANOVA; n = 4-11). (D) PLC numbers in spleens of PBS (Ctrl) or virus (CMV)-injected Rag1–/– (R/Rag, left panel), wild-type (R/wt), and NK-depleted wt (NK-dep, right panel) Eμ-ret pups at 10 dpi (Mann-Whitney; R/Rag1, n = 10 PBS and 10 MCMV; 1-way ANOVA; n = 8 PBS, 8 CMV, and 7 CMV + NK-depleted; pooled results from 3 independent experiments). All data presented as mean ± SEM (∗∗∗P < .001; ∗∗∗∗P < .0001). ns, not significant (nonsignificant comparisons in graphs A and C are not labeled).
