Phenotypic correction after WU-106/AMD3100 mobilization and in vivo HSC prime editing: spleen and liver analyses. (A) Spleen size (left panel) and spleen weight relative to the body weight (right panel). Each symbol represents an individual mouse. Two-way ANOVA with Bonferroni posttest for multiple comparisons was used. Statistical analysis was performed using GraphPad Prism version 10.0.3. ∗∗∗P ≤ .001, ∗∗P ≤ .01, ∗P ≤ .05. (B) Hemosiderosis: spleen and liver sections stained with Perl Prussian blue. Iron deposition is shown as the cytoplasmic blue pigments of hemosiderin in the spleen tissue sections. The scale bars are 20μm.