Lrba, Lyst, and Nbeal1 are positive regulators of BM-HSPC engraftment. (A) qRT-PCR of Nbea, Lrba, Lyst, Nbeal1, Nbeal2, Wdfy3, and Wdfy4 mRNA expression in the hematopoietic hierarchy of adult BM. (B) qRT-PCR of Nbea, Lrba, Lyst, Nbeal1, Nbeal2, Wdfy3, and Wdfy4 expression in Nbea^+/+ and Nbea^–/– E14.5 FL-HSCs. (C-E) Nbea^+/+ and Nbea^−/− CD45.2⁺ HSPCs recovered from CD45.1⁺/CD45.2⁺ recipients of E14.5- CD45.2⁺ FL-HSPCs 4 months after transplant were transduced with shRNAs targeting Lrba, Lyst, Nbeal1, or Nbeal2 and transplanted together with CD45.1⁺ HSPCs into CD45.1⁺/CD45.2⁺ (ratio 3:2). (C) Experimental schematic. (D) Percentage of mCherry⁺CD45.2⁺ PB after transplant of recipients of shRNA treated cells normalized to the average of control recipients. (E) Percentage of mCherry⁺CD45.2⁺ chimerism in the BM HSPCs at 20 weeks after transplant. Data are represented as mean ± SEM. ∗/#/•/∇P < .05; ∗∗/##/••/∇∇P < .005; ∗∗∗/###/•••/∇∇∇P < .001, relative to recipients of control-shRNA HSPCs. ∗ refers to Lrba-shRNA; # refers to Nbeal1-shRNA; • refers to Nbeal2-shRNA; and ∇ refers to Lyst-shRNA.