TNF exacerbates Tet2–mutant T-cell remodeling with age. Peripheral whole blood of recipient mice was collected 8 weeks after BMT and analyzed by flow cytometry for the absolute and relative counts of circulating lymphocytes. (A-C) Absolute and relative (as a percentage of total CD45⁺ leukocytes) counts of total T cells (A), CD8⁺ T cells (B), and CD4⁺ T cells (C). (D-E) Absolute and relative counts of CD8⁺ (D) and CD4⁺ naïve T cells (E) as a percentage of total CD8⁺ and CD4⁺ T cells, respectively. (F) Absolute counts of circulating CD8+ naïve T cells expressing CD183. (G-I) Relative count of CD8⁺ T_EM (G), T_VM (H), and T_CM cells (I). (J-M) Relative count of CD4⁺ T_VM (J), T_EM (K), and T_CM cells (L). (M) Myeloid-to-lymphoid ratio. (N) Leukocyte summary. Significance was assessed by a 2-way ANOVA with Tukey multiple comparisons test. Letters in the orange and blue columns denote significant differences (P ≤ .05) in the group means of CD45.2 or CD45.1 alleles, respectively. For all variables with the same letter, the difference between the means is significantly different. If 2 variables have different letters or no letters, they are not significantly different. Black bars with asterisks denote group differences, inclusive of both CD45.1 and CD45.2 alleles. ∗P ≤ .05; ∗∗P ≤ .01; ∗∗∗P ≤ .001; ∗P ≤.0001. Abbreviations: T_EM, effector memory T cells; T_VM, virtual memory T cells; T_CM, central memory T cells; NK, natural killer; NKT, natural killer T cells.