PCs and B cells in SMM and WM of patient P4. (A) Violin plot of BCR clonotypes of sample P4, representing the expression of heavy and light chains in each clonotype of SMM and WM cells. Clonotype 1 is represented in red, clonotype 2 in green, and polyclonal cells in blue. (B) CNAs analyzed by InferCNV pipeline, defining 3 different clonotypes: subclone 1 (MM PCs) is represented in light blue, subclone 2 (WM memory B cells) in green, and subclone 3 (WM PCs) in violet. (C) Violin plot representing the expression of heavy and light chain in each MM and WM subclones and in TME, represented in gray. (D-E) Color coded map of clonotypes and MM and WM subclones distribution in the UMAP space, with the same color code of panel B. TME is shown in gray. (F) Violin plot showing the expression of selected marker genes by subclones. (G-H) Pathway enrichment analysis, showing the comparison of transcriptional profiles of MM PCs vs WM PCs (G) and WM memory B cells vs WM PCs (H) (.1 as P value cutoff; Padj method by Benjamini-Hochberg; see “Materials and Methods”).