Change in plasma iron and transferrin saturation for each individual between first blood sample drawn at study enrollment and second blood sample drawn for a repeat measurement of plasma iron or transferrin saturation. For a total of 32 141 individuals, a second blood sample for a repeat measurement of iron and/or transferrin saturation was obtained at a median of 10 years (IQR, 9.6-10.7; range, 0.7-15.8) after the first blood sample. Median modulus value (nonnegative value) for change in plasma iron was 3.2 μmol/L (IQR, 1.7-5.9). For transferrin saturation median modulus value (nonnegative value) for change was 6% (IQR, 3%-10%). Importantly, all main analyses on risk of infections were based on the blood samples drawn at the day of study enrollment (first blood sample), except for supplemental Figure 4 in which the second blood sample for repeat measurements of iron and transferrin saturation was used to model risk of infection. No individuals had repeat measurements of ferritin. IQR, interquartile range.