Identification of subpopulations within the different stages of B to PC differentiation. (A) Seurat k-nearest neighbors clustering identified 7 clusters: 1 cluster corresponding to MBC and 2 clusters for each stage of prePB, PB, and PC. (B) Identification of quiescent cells (G1) and proliferative cells (S and G2M) using the Seurat cell-cycle scoring. (C) mRNA expression of CDC20, CDK1, MKI67 and PCNA involved in cell cycle. High and low expression were represented in dark blue and in yellow, respectively. (D) Cell-cycle distribution of each stage. (E) Heat map displaying the average expression of selected genes in clusters identified in panel A.