Alterations in the renal microvascular EPCR distribution in SS mice after multiheme-induced CKD. (A) Representative images from immunofluorescence staining indicating a lack of EPCR in the renal endothelium marked with CD31-positive staining. (B) Quantitation of staining intensity (EPCR/CD31 ratio), indicating significant deterioration of renal endothelial EPCR in heme-challenged mice (n = 6; M, 3; F, 3). (C-D) Higher concentrations of EPCR in the plasma (C) and urine (D) detected in the heme-challenged SS mice compared with those challenged with vehicle (n = 8; M, 4; F, 4). (E) Quantitation of thrombin accumulation in SS mouse kidneys after a multiheme challenge (n = 6; M, 3; F, 3). ∗P < .05; ∗∗P < .01 (unpaired Student t test).