The combined deletion of PDK2/4 inhibits acute DVT. (A) Thrombus incidence in male PDK2/4^–/– mice and WT mice 48 hours after IVC stenosis; n = 8 mice per group. Statistical analysis was performed using Fisher exact test. (B) The representative image of IVC thrombus harvested from PDK2/4^–/– mice and WT mice 48 hours after IVC stenosis. The bar graphs show quantified mean thrombus weight and thrombus length. Values are mean ± SEM, n = 8 mice per group. Statistical analysis was performed using Mann-Whitney U test. (C) The level of Cit-H3 was measured in the thrombus isolated from PDK2/4^–/– mice and WT mice 48 hours after IVC stenosis. A representative western blot for Cit-H3 is shown. Ponceau S (Ponc. S) staining was used as a loading control. The bar graphs show densitometry analysis of immunoblots. Values are mean ± SEM, with n = 4 mice per group. Statistical analysis was performed using Mann-Whitney U test.