A mouse model of Npm1^cA AML that develops MSs reveals an EMT-like signature. (A) Example of an Npm1^cA animal that has developed MSs. (B) Representative H&E and IHC for TWIST1. Black bars indicate 50 μm. (C) GSEA comparing MS and AML BM samples from mice, with an EMT-like signature being the top hit based upon NES. RNA was isolated from freshly collected tissue. Significantly differentially expressed gene sets were identified as having a P value <.01 and FDR <5%. (D) Volcano plot indicating the expression of TWIST1. Significantly differentially expressed genes were identified by a log₂fold change of <1 or >1 and a Padj <.05. (E) RT-qPCR data for Twist1 expression in cKit⁺ cells from AML BM and MS, relative to the cKit^– cells from each tissue. RT-qPCR, Reverse-Transcriptase Quantitiative PCR.