Transcriptomics of matched human samples identifies an EMT-like signature. (A) GSEA comparing MS and AML BM samples, with an EMT-like signature being the top hit based upon normalized enrichment score (NES). RNA was extracted from FFPE blocks. Significantly differentially expressed gene sets were identified as having a P value <.01 and FDR <5%. (B) Volcano plot indicating the expression of different transcription factors associated with EMT. Significantly differentially expressed genes were identified by a log₂fold change of <1 or >1, and an Padj <.05. (C) Representative H&E and immunohistochemistry (IHC) for CD45 (white cell marker) and TWIST1. Black bars indicate 50 μm. All images derived from patient number 2 (supplemental Table 1). FDR, false discovery rate; GSEA, Gene Set Enrichment Analysis; H&E, hematoxylin and eosin.