Genotype–grouped CD14⁺ monocyte vs control comparisons highlight inflammatory cell-extrinsic effects of CH mutations. (A) Violin plots displaying expression of FN1 and FCER2 in CD14⁺ monocytes from controls and CD14⁺ monocytes from patients with TET2 mutations. (B) Violin plots displaying expression of IFITM2 and ADGRE5 in CD14⁺ monocytes from controls and CD14⁺ monocytes from patients with DNMT3A mutations. (C) Volcano plot showing results of differential expression analysis comparing CD14⁺ monocytes from patients with TET2 mutations with CD14⁺ monocytes from controls, colored by biological pathway. (D) Volcano plot showing results of differential expression analysis comparing CD14⁺ monocytes from patients with DNMT3A mutations with CD14⁺ monocytes from controls, colored by biological pathway. (E) GSEA based on results from differential expression analysis comparing CD14⁺ monocytes from patients with TET2 mutations with CD14⁺ monocytes from controls. (F) GSEA based on results from differential expression analysis comparing CD14⁺ monocytes from patients with DNMT3A mutations with CD14⁺ monocytes from controls. (G) Heat map showing predicted outgoing signaling from CD14⁺ monocytes from patients with TET2 mutations and from controls for pathways involved in inflammatory response and immune cell migration, as determined by CellChat (∗P < .05/7). (H) Heat map showing predicted outgoing signaling from CD14⁺ monocytes from patients with DNMT3A mutations and from controls for pathways involved in inflammatory response and immune cell migration, as determined by CellChat (∗P < .05/7). GSEA, gene set enrichment analysis.