Mutant CD14⁺ monocytes from 4 patients with TET2 and 2 patients with DNMT3A highlight inflammatory cell-intrinsic effects of CH mutations. (A) Violin plots displaying expression of cytokines in control CD14⁺ monocytes and TET2 mutant CD14⁺ monocytes. (B) Violin plots displaying expression of cytokines in control CD14⁺ monocytes and DNMT3A mutant monocytes. (C) GSEA based on results from differential expression analysis comparing TET2 mutant CD14⁺ monocytes with control CD14⁺ monocytes. (D) GSEA based on results from differential expression analysis comparing DNMT3A mutant CD14⁺ monocytes with control CD14⁺ monocytes. (E) Quantification of phospho-flow cytometry displaying pSTAT3(Y705)⁺ in the CD33⁺ gate of large VAF TET2 CH samples (>25%; n = 3) compared with both low VAF (<25%; n = 3) and control samples (n = 4) (∗∗P < .01 by analysis of variance with Tukey's Honestly Significant Difference). (F) With IL-6 stimulation condition (similar to E). (G) Pearson correlation of %pSTAT3(Y705)⁺ in the CD33⁺ gate and VAF after IL-6 stimulation. For samples with multiple mutations, the highest VAF value was selected for the analysis. GSEA, gene set enrichment analysis.