RORα binds to the EPO promoter to regulate its gene expression. HepG2 cells were treated with DMOG or SR1078 or combination for 24 hours and EPO expression was evaluated for (A) relative mRNA expression by qRT-PCR and (B) the protein levels in the cell culture supernatant by ELISA. (C) qRT-PCR analysis of EPO in HepG2 cells ectopically expressed with RORα. (D) O.E of RORα in HepG2 cells determined with the qRT-PCR analysis. (E) ChIP analysis of RORα binding to the human EPO promoter region using chromatin extracted from HepG2 cells treated with SR1078 for 24 hours. (F) EMSA with RORα protein by using WT and mutant radiolabeled probes. Data are representative and mean ± SD from 3 independent experiments. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001, as indicated.