EPO is a direct target gene of Rev-erbα. (A) Pictorial representation of a RREs on the human EPO promoter. (B) ChIP analysis of Rev-erbα binding to the human EPO promoter region using chromatin extracted from HepG2 cells treated with GSK4112 for 24 hours. (C) EMSA with Rev-erbα protein by using WT (containing h-RRE1 and h-RRE2) and mutant radiolabeled probes. Data are representative and mean ± SD from 3 independent experiments. ∗P < .05; ∗∗P < .01, as indicated.