Map of variant frequency across EBV genomes. (A) Identification and frequency by region and histology of all variants identified in 1376 EBV genomes meeting predetermined quality metrics. (B) Comparison of variant detection in standard NGS and duplex sequencing. Bar colors represent the nonsynonymous variant type detected. (C) Comparison of frequency of previously identified disease–associated nonsynonymous mutations in NKTCL and NPC. Variants previously associated with NPC are starred, all others were previously associated with NKTCL.