Increased bleeding tendency for f10^low mice in tail-clip model. (A) The terminal 3 mm of the tail were amputated from f10^WT mice (blue), f10^low mice (red), f10^low mice that received FX (50 U/kg) 5 minutes before injury (green), and f8^KO mice (f8^KO; gray). Blood loss was monitored for 30 minutes, and the amount of shed blood was measured. Statistical analysis was performed using 1-way analysis of variance (ANOVA) with Tukey correction for multiple comparisons. (B) Number of mice that made (dark gray) or not (pale gray) a primary bleeding stop after tail amputation. The number of mice with a primary stop was significantly reduced in f10^low mice than in f10^WT and f8^KO mice. Statistically significant difference in the number of mice with primary stop was assessed using a χ² test for multiple groups. (C) Representative bleeding profiles of f10^WT mice, f10^low mice, f10^low mice that received FX (50 U/kg) 5 minutes before injury, and FVIII-deficient mice.